Remove Pet Urine Odor From Carpet Padding | Professional Guide
If you’ve cleaned your carpet multiple times but still smell pet urine, especially on humid days, the problem isn’t in your carpet fibers—it’s deep in the padding underneath. Surface cleaning with home carpet cleaners won’t eliminate the odor because these methods can’t reach where the real contamination lives. To truly remove pet urine from carpet padding, you need specialized sub-surface extraction techniques, professional-grade enzymatic treatments applied directly into the padding, or in severe cases, complete padding replacement.
We understand how frustrating this is. You love your pets, but coming home to that unmistakable ammonia smell is unbearable. You’ve tried every product at the store, maybe even hired a service that promised results. Yet every time it gets warm or humid, that odor comes right back.
Why Surface Cleaning Doesn’t Work

Here’s what most pet owners don’t realize, when your dog or cat has an accident, that urine doesn’t just sit on top of the carpet fibers. Within seconds, it soaks downward through your carpet and into the padding below. The carpet padding acts exactly like a sponge, absorbing and trapping the urine.
The visible stain on your carpet surface represents only a fraction of the actual contamination. When urine reaches the padding, it spreads horizontally, creating a contaminated area that’s typically five to ten times larger than the visible surface stain. In severe cases, the urine continues down to the subfloor beneath everything else.
The Science Behind the Persistent Smell
Fresh urine contains water, uric acid, urea, proteins, and other organic compounds. As it sits in your carpet padding, bacteria begin breaking it down. This creates ammonia that sharp, nose-burning smell you recognize. Worse, uric acid forms crystals that aren’t water-soluble, which means regular cleaning can’t dissolve them.
These crystals are hygroscopic, meaning they absorb moisture from the air. This is why the smell gets worse on humid days or when you run the heat—the crystals are reactivating and releasing odor. Cat urine is even more challenging because it contains mercaptans, sulfur-based compounds that create an especially pungent odor.
Why Your DIY Methods did not work

Baking soda and vinegar can help with fresh surface accidents, but they can’t penetrate deep enough to reach urine in the padding. Retail enzymatic cleaners are a step in the right direction, but they’re not concentrated enough and most of the product never reaches the padding where it’s needed.
Steam cleaning or rental carpet cleaners actually make things worse. Heat sets protein-based stains permanently by bonding them to carpet fibers. Plus, rental machines have weak suction compared to professional equipment—you’re adding water but not extracting it effectively, which pushes urine deeper and spreads contamination wider.
How Professional Pet Urine Removal Actually Works
Professional carpet cleaning companies use completely different equipment and techniques designed specifically to reach carpet padding and eliminate contamination at the source.
UV Light Inspection

We start with UV black light inspection to find all contaminated areas, including dried stains from months ago. Pet urine contains phosphorus that glows under ultraviolet light even when completely dry and invisible. We typically discover many more spots than homeowners realized existed.
Sub-Surface Extraction

This is where professional treatment differs dramatically from DIY methods. We use specialized tools like the Water Claw that inject cleaning solution under pressure directly into the carpet padding, then extract it along with the dissolved urine using powerful truck-mounted vacuum systems.
Our equipment generates 400-800 PSI of vacuum pressure compared to rental machines’ 100 PSI or less. We flush each contaminated area multiple times until the extracted liquid runs clear. This sub-surface extraction process literally pulls the urine out of the padding rather than just cleaning the surface.
Professional Enzymatic Treatment

After extraction removes bulk contamination, we inject commercial-grade enzymatic solutions directly into the padding. These products are significantly stronger than retail versions. The enzymes need 24-72 hours to work, actively consuming organic compounds and dissolving those stubborn uric acid crystals that regular cleaning can’t touch.
Final Extraction and Neutralization
We perform thorough hot water extraction to rinse away enzyme residues and any remaining contamination. Then we apply pH-balancing treatments and odor neutralizers not deodorizers that mask smells, but products that chemically neutralize odor molecules.
When Padding Replacement Is Necessary

Sometimes padding is too contaminated to save. This happens when pets repeatedly urinate in the same spot over weeks or months. We carefully pull back the carpet, remove contaminated padding sections, treat the subfloor if necessary, and install new moisture-barrier padding designed to resist future accidents.
Signs You Need Professional Help
You should call professionals when you notice:
- Persistent odor that returns after cleaning, especially worse on humid days
- Multiple cleaning attempts have failed to eliminate the smell
- Old, dried stains that have been there for weeks or longer
- Carpet feels damp in spots that weren’t recently cleaned
- Discoloration from underneath showing through the carpet surface
- Strong ammonia smell rather than just “pet smell”
Cat urine almost always requires professional treatment due to its chemical complexity. If you’ve already tried DIY methods without success, continuing to spend money on retail products is just throwing good money after bad.
DIY vs Professional: Quick Comparison
| Factor | DIY Methods | Professional Treatment |
|---|---|---|
| Equipment Power | 100 PSI or less | 400-800 PSI vacuum pressure |
| Penetration Depth | Surface fibers only | Reaches deep into padding |
| Product Strength | Retail enzymatic cleaners | Commercial-grade enzymes |
| Success Rate (Old Stains) | 10-20% | 85-95% |
| Typical Cost (Failed Attempts) | $150-200+ in products | $100-500 one-time service |
| Time to Results | Multiple attempts over weeks | 24-72 hours |
| Risk of Damage | High (heat setting, over-wetting) | Minimal (trained technicians) |
| Padding Access | No access | Direct injection & extraction |
The Cost Reality

Most homeowners spend $150-200 on failed DIY attempts before calling professionals. Our basic professional treatment typically costs $100-200 for small areas less than you’d waste on products that don’t work, and we actually solve the problem.
More extensive sub-surface extraction runs $200-500 depending on severity. Padding replacement costs $500-1,500 for affected areas. While this seems expensive, compare it to full carpet replacement at $3,000-8,000. Professional treatment extends your carpet’s life and protects your investment.
Quick Prevention Tips
If you catch an accident immediately (within 5 minutes), aggressive blotting can prevent padding contamination. Stack thick layers of paper towels on the spot and stand on them to absorb as much liquid as possible. Repeat with fresh towels until they stay dry.
If you’re installing new carpet, consider moisture-barrier padding that resists liquid penetration. These pads give you extra time to clean accidents before they reach the subfloor.
Always address underlying pet health or behavioral issues. Sometimes persistent accidents indicate medical conditions like urinary tract infections or diabetes that need veterinary attention.
Don’t Live With Pet Urine Odor Any Longer
Pet urine in carpet padding isn’t just an odor nuisance it’s a health concern that affects your family’s quality of life. The ammonia and bacteria in contaminated padding can trigger allergies and respiratory issues. Every day you wait, the problem gets worse as contamination spreads and bacteria multiply.
Professional treatment is an investment in your home and comfort. It protects your flooring investment, creates a healthier environment, and gives you back the clean-smelling home you deserve.
You shouldn’t have to choose between the pets you love and a fresh, clean home. With the right professional treatment, you can have both.
Professional Pet Urine Removal in Ames, Iowa

At A-1 Carpet Cleaning, we specialize in complete pet urine removal from carpet padding—not just surface cleaning. Our experienced technicians use professional-grade equipment and proven techniques to eliminate odors permanently.
We offer:
- Free UV light inspection to identify all contaminated areas
- Sub-surface extraction with truck-mounted systems
- Commercial-grade enzymatic treatments
- Carpet padding replacement when necessary
- 100% satisfaction guarantee
- Same-day emergency service available
Don’t spend another day living with pet urine odor or waste more money on DIY solutions that don’t work. Let us solve your problem permanently with professional treatment that actually reaches the source of contamination.
📍 Serving Ames, Iowa & surrounding areas
📞 Call A-1 Carpet Cleaning: 515-432-7500
🌐 Visit us: https://a-1-carpetcleaning.com/
Get your fresh-smelling home back today. Contact us for a free inspection and see the A-1 difference!